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Image / CIL:9969, Rattus, permanent cell line cell, permanent cell line cell, Kidney

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Title
CIL:9969, Rattus, permanent cell line cell, permanent cell line cell, Kidney
Creator
Satpute-Krishnan, Prasanna
Contributor
Satpute-Krishnan, Prasanna
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Creative Commons Public Domain Dedication
Constraint(s) on Use: This work may be used without prior permission.
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description
Normal Rat Kidney (NRK) cells grown in culture expressing Galactosyl Transferase-YFP (GalT-YFP) and p58-CFP. This file is the merged CFP/YFP time series demonstrating the organization of the early secretory pathway (Golgi and intermediate compartment). Images were collected on a Zeiss Spinning Disk confocal as a single z slice over time with CFP imaged using 445ht and YFP imaged using 515ht and a 63X oil objective. Images were collected every 2 minutes. Exposure time: 515 was 50ms; 445 was 200ms: for both channels, the gain was 3, the camera intensification was 200, the laser neutral density was 10.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Satpute-Krishnan, Prasanna (2021). CIL:9969, Rattus, permanent cell line cell, permanent cell line cell, Kidney. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0V40TCF
Type
image
Identifier
ark:/20775/bb1167553t
Language
No linguistic content
Subject
Constitutive secretory pathway
NRK
Endoplasmic reticulum-Golgi intermediate compartment
Permanent cell line cell
Kidney
Golgi apparatus
Rattus
Cell Image Library Group ID: 2315

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