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Description
Cultured retinal pigment epithelial cells immunofluorescently labeled for transferrin receptor (TfnR) (green) and nucleus (blue). The cells were fixed in 2% PFA and 0.5% Triton X-100 for 2 minutes followed by post-fixation 4% PFA. TfnR was detected with H68.4 primary antibody and secondary FITC antibody. The nucleus was detected with DAPI staining. Images were collected on an Olympus IX-71 epifluorescence microscope using a 100X 1.4 NA objective with 4.500ms exposure for TnfR and 50ms exposure for DAPI (67nm/pixel). Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Liu, Allen; Schmid, Sandra L. (2021). CIL:10104, Homo sapiens, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02J6B1F
Type
image
Identifier
ark:/20775/bb1304079t
Language
No linguistic content
Subject
ARPE-19 Epithelial cell Transferrin receptor Nucleus Homo sapiens
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