Creative Commons Public Domain Dedication Constraint(s) on Use: This work may be used without prior permission. Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description
Phosphorus has a characteristic electron energy loss with an edge at 160 eV. The Zeiss 902 electron microscope permits images to be recorded at different energy losses which can be digitized and processed to yield a net phosphorus image. For nuclear chromatin, phosphorus is primarily located in DNA, allowing its distribution in chromatin fibers to be mapped. Starfish sperm chromatin, which has clearly defined chromatin fibers was fixed, embedded in Lowicryl at low temperature, thin sections prepared and imaged unstained. The 4 grouped images were recorded with a Zeiss 902 energy filtered TEM at 80KV using 5eV windows centered on energy loss values of 170 (this image), 160, 118, and 104eV. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Horowitz-Scherer, Rachel; Woodcock, Christopher (2021). CIL:755, Patiria miniata, sperm. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0H41QPH
Type
image
Identifier
ark:/20775/bb14744432
Language
No linguistic content
Subject
DNA packaging Fertilization Nuclear chromatin Sperm Patiria miniata Cell Image Library Group ID: 8086
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
