Image / CIL:36736, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist

View source image on contributor's website.

Get Citation

Have a question about this item?

Contact Owner

Item information. View source record on contributor's website.

Title: CIL:36736, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
Creator: Allen, Richard
Contributor: Allen, Richard
Date Created and/or Issued: 2021
Contributing Institution: UC San Diego, Research Data Curation Program
Collection: Cell Image Library
Rights Information: Creative Commons Public Domain Dedication
Constraint(s) on Use: This work may be used without prior permission.
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description: In order to study all stages of DVs in the same cell we used a protocol of sequentially exposing cells to different sizes of latex beads interspersed with washes: for example, 3 minutes in 0.1µm beads, wash 3 min, 3 minutes in 0.3µm beads, wash 3 min, 3 minutes in 0.6µm beads, wash 3 min, 3 minutes in 0.8µm beads, wash 3 min and finally 3 minutes in 1.1µm beads followed by fixing the cells. Thus the 1.1, 0.8, 0.6, 0.3 and 0.1µm bead-containing vacuoles will be 0-3 min, 6-9 min, 12-15 min, 18-21 min and 24-27 minutes old, respectively. The electron micrograph shown here was of a DV-II that was surrounded with docked secondary lysosomes. Secondary lysosomes only recognize and dock at the DV-II membrane. They do not dock with DV-I or DV-III vacuoles. TEM taken on 2/27/81 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 7,000X. Bar = 0.5µm.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Allen, Richard (2021). CIL:36736, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0V123QZ
Type: image
Identifier: ark:/20775/bb15431593
Language: No linguistic content
Subject: Digestive system process
Ciliated Protist
Eukaryotic Protist
Secondary lysosome
Food vacuole
Eukaryotic cell
Cell by organism
Paramecium multimicronucleatum

About the collections in Calisphere

Learn more about the collections in Calisphere. View our statement on digital primary resources.

Copyright, permissions, and use

If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.

Share your story

Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.