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CIL:50585, fine structure and quantified the distribution of melanopsin-expressing retinal ganglion cell (ipRGC) projections within multiple brain regions

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Title: CIL:50585, fine structure and quantified the distribution of melanopsin-expressing retinal ganglion cell (ipRGC) projections within multiple brain regions
Creator: Bushong, Eric
Deerinck, Thomas J
Ellisman, Mark
Ellisman, Maya
Hatori, Megumi
Ju, Suyeon
Kim, Keun-Young
Le, Hiep
Lev-Ram, Varda
Liu, Cindy
Mure, Ludovic
Panda, Satchidananda
Panda, Sneha
Perez, Alex
Phan, Sébastien
Rios, Luis
Yoon, Sanghee
Contributor: Bushong, Eric
Deerinck, Thomas J.
Ellisman, Mark
Ellisman, Maya
Hatori, Megumi
Ju, Suyeon
Kim, Keun-Young
Le, Hiep
Lev-Ram, Varda
Liu, Cindy
Mure, Ludovic
Panda, Satchidananda
Panda, Sneha
Perez, Alex
Phan, Sébastien
Rios, Luis
Yoon, Sanghee
Date Created and/or Issued: 2021
Contributing Institution: UC San Diego, Research Data Curation Program
Collection: Cell Image Library
Rights Information: Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact: UC Regents
Description: Using a variant of miniSOG, a genetic probe for correlated light and electron microscopy (CLEM), we determined the form, fine structure and quantified the distribution of melanopsin-expressing retinal ganglion cell (ipRGC) projections within multiple brain regions. The method developed labels the entire neuron, including its axonal arbors in retinorecipient brain regions. Here the ‚Äòsynapto-projectome‚Äô of these cells was charted in the suprachiasmatic nucleus (SCN), olivary pretectal nucleus (OPN), and subregions of the lateral geniculate. Each ipRGC axonal arbor synapsed on multiple target neurons in the SCN and OPN. Although the only direct retinal input to the OPN and SCN is from ipRGCs, the terminal arbors and boutons were different in each brain region. Also, a network of dendro-dendritic chemical synapses (DDCS) was discovered in the SCN; with ipRGC axons preferentially targeting the DDCS-linked cells. Methods used for this analysis should facilitate other CLEM studies of brain circuits at high resolution.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Kim, Keun-Young; Rios, Luis; Le, Hiep; Perez, Alex; Phan, Sébastien; Bushong, Eric; Deerinck, Thomas J.; Liu, Cindy; Ellisman, Maya; Lev-Ram, Varda; Ju, Suyeon; Panda, Sneha; Yoon, Sanghee; Mure, Ludovic; Hatori, Megumi; Ellisman, Mark; Panda, Satchidananda (2021). CIL:50585, fine structure and quantified the distribution of melanopsin-expressing retinal ganglion cell (ipRGC) projections within multiple brain regions. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0HD7THJ
Type: image
Identifier: ark:/20775/bb1610940h
Language: No linguistic content
Subject: Opn4Cremouse brain tissue
MiniSOG in melanopsin retinal ganglion cells
Neurites
Mus musculus

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