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Description
Primary endothelial cells from transgenic mouse expressing myosin heavy chain 2A fused to mCherry (red), transfected with GFP-myosin (green) regulatory light chain expression vector and demonstrate the role of myosin in cell division. Cells are embedded in 3D collagen gels. mage Name: myosin cell division timelapse Biological Source: Primary mouse (mus musculus) aortic endothelial cell. 2D images over time. 0.1274 micron/pixel, 520x490 pixels, 20 seconds interval between timepoints.Time shown is in minutes:seconds. Images collected with a spinning disk confocal on a Nikon TE1000 with a 60X 1.2NA water objective using 400 ms exposure for mCherry and 200 ms for GFP. See Fischer et al for details regarding preparation. http://www.ncbi.nlm.nih.gov/pubmed/19185493 Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Fischer, Robert S.; Waterman, Clare M. (2021). CIL:7329, Mus musculus, blood vessel endothelial cell, primary cell line cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J06972KH
Type
image
Identifier
ark:/20775/bb1679421t
Language
No linguistic content
Subject
Myosin II binding Cell division Actomyosin contractile ring contraction Blood vessel endothelial cell Myosin II complex Primary cell line cell Mus musculus
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