Image / CIL:13112, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist

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Title: CIL:13112, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
Creator: Allen, Richard
Contributor: Allen, Richard
Date Created and/or Issued: 2021
Contributing Institution: UC San Diego, Research Data Curation Program
Collection: Cell Image Library
Rights Information: Creative Commons Public Domain Dedication
Constraint(s) on Use: This work may be used without prior permission.
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description: Collecting canal of the contractile vacuole complex. Normally the collecting canal is expanded when the CV is in systole. In this micrograph the collecting canal is empty so that its two sides are flattened against each other. Presumably the CV is in diastole (filling phase). The ribbon of microtubules is split into 10 groups of two tubules each so the ribbon has 18 to 20 microtubules at this level. The smooth spongiome is connected to the collecting canal and bundles of decorated spongiome form the peripheral layer of the radial arm. The decorations consist of 2 closely set globular units forming a helix around the tubule. In this micrograph the globular units are outlined with more electron dense material so it appears there are 3 threads making up a helix. TEM taken on 12/9/70 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 24,000X. Bar = 0.2µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:13096) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Allen, Richard (2021). CIL:13112, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0BR8RBR
Type: image
Identifier: ark:/20775/bb16795548
Language: No linguistic content
Subject: Contractile vacuole organization
Microtubule-based process
Microtubule associated complex
Eukaryotic cell
Contractile vacuole
Eukaryotic Protist
Ciliated Protist
Cell by organism
Cytoplasm
Collecting canal
Paramecium multimicronucleatum
Cell Image Library Group ID: 3939

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