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Description
Amphibian oocytes have many copies of rRNA genes that are actively transcribed. Newt ocyte nuclei were dissected, allowed to swell in a low ionic strength high pH buffer, centrifuged through a sucrose-formaldehyde cushion onto EM grids coated with carbon films, and stained with ethanolic phosphotungstic acid producing a positive stain. In this survey image, the transcribed genes appear tree-like as the closely packed polymerases traverse the gene, and the nascent rRNA transcripts elongate until detached at the termination point. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Woodcock, Christopher (2021). CIL:563, Notophthalmus viridescens, oocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0B857BK
Type
image
Identifier
ark:/20775/bb1713346h
Language
No linguistic content
Subject
Ribosomal RNA transcription RNA metabolic process Oocyte Nuclear chromosome Notophthalmus viridescens Cell Image Library Group ID: 10245
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