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Description
This is Video S1 and it corresponds to Figure 2A. It shows that local blebbistatin micropipetting, but not DMSO, increases spine formation and extension (arrowheads). Equal volumes of blebbistatin and DMSO were used. Images were acquired every ~6 seconds using confocal microscopy. 20 frames/sec shown. A DIV 7 cortical neuron expressing DsRed2 was locally micropipetted with either DMSO or 100 mM blebbistatin at the indicated times. Note the increase in the fraction of spines that appear and extend in response to blebbistatin. Arrowheads indicate either nascent or elongating spines. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Hodges, Jennifer L.; Newell-Litwa, Karen; Asmussen, Hannelore; Vicente-Manzanares, Miguel; Horwitz, Alan Rick (2021). CIL:37154, Rattus, CNS neuron (sensu Vertebrata). In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0ZG6SB7
Type
image
Identifier
ark:/20775/bb1714330b
Language
No linguistic content
Subject
Myosin II light chain binding Dendritic spine morphogenesis Dendritic spine development ATPase activity Dendritic spine CNS neuron (sensu Vertebrata) Dendrite Dendritic shaft Cytoplasm Rattus Cell Image Library Group ID: 9258
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