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Description
This is IMAGE #1 (day 1) of a time lapse series contained with in this image group. It is a group of radial glia and neurons from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47. Visible in the superficial z-stack layers are autofluorescent pigment cells. The images were acquired daily over 3 days and show dendritic arbor development and morphological plasticity of the dendritic arbor with branch additions and retractions. The cells were transfected with in vivo electroporation of plasmid DNA encoding eYFP ~24 hours before the first image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Bestman, Jennifer; Cline, Holly (2022). CIL:36955, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RX9BWS
Type
image
Identifier
ark:/20775/bb1850345m
Language
No linguistic content
Subject
Morphogenesis of a branching structure Neuron development Dendrite CNS neuron (sensu Vertebrata) Neuronal cell body Cytoplasm Axon Xenopus laevis Cell Image Library Group ID: 9198
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