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Image / CIL:9161, Homo sapiens, permanent cell line cell, epithelial cell

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Title
CIL:9161, Homo sapiens, permanent cell line cell, epithelial cell
Creator
Sengupta, Prabuddha
Contributor
Sengupta, Prabuddha
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Creative Commons Public Domain Dedication
Constraint(s) on Use: This work may be used without prior permission.
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description
Sialyl Transferase-EGFP transfected HeLa cells demonstrating the redistribution of the Golgi Apparatus to the Endoplasmic Reticulum upon treatment with Brefeldin A (BFA). Images were collected as a 4D time series (z-stack over time). z-stacks of 48 individual timepoints are included in this collection of files as well as the movie SiEGFP_BFA.avi, which is the maximum projection of the z-stacks at the individual time points. BFA was added according to the following protocol: Timepoint1: Before addition of BFA 0seconds (8 Z-slices at 1 um apart) Timepoint2: After addition of BFA 130seconds (8 Z-slices at 1 um apart) Timepoint3: 238 seconds (11 Z-slices at 1 um apart) Timepoint3 thru Timepoint48: Images captured every 5 seconds (11 Z-slices at 1 um apart at each time point) Images were collected on a Carl Zeiss spinning disk confocal with a 63X 1.4 NA objective with 250 ms exposures at 5 s intervals.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Sengupta, Prabuddha (2021). CIL:9161, Homo sapiens, permanent cell line cell, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0542MQ0
Type
image
Identifier
ark:/20775/bb1918392m
Language
No linguistic content
Subject
HeLa
ER to Golgi vesicle-mediated transport
Endoplasmic reticulum
Epithelial cell
Golgi apparatus
Permanent cell line cell
Homo sapiens
Cell Image Library Group ID: 7926

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