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Description
Purified rabbit skeletal muscle myosin in 0.6M KCl was deposited onto mica flakes, quick-frozen by contact with a liquid helium- cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 11 degrees and visualized in a JEOL 100CX2 transmission electron microscope at 100 kev. Images were recorded on Kodak 4489 film at 100,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED. Note the classic appearance of the individual molecules with two globular heads (myosin subfragment 1) and the long rod-like tail. RIGHT image of a stereo pair. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Ip, Wallace (2021). CIL:832, Oryctolagus cuniculus, skeletal muscle cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WW7GZG
Type
image
Identifier
ark:/20775/bb2088786v
Language
No linguistic content
Subject
ATPase activity, coupled to movement of substances Skeletal muscle contraction Skeletal muscle cell Cytoskeleton Oryctolagus cuniculus Cell Image Library Group ID: 1847
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