Under copyright Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work. Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
Time lapse recording showing the development of neuron polarity in a hippocampal neuron growing in vitro. This recording captures not only the formation of the axon (the longest process, oriented downward in the last frame), but also accelerated development and outgrowth of the dendritic arbor due to treatment with BMP-7, a growth factor that has been found to selectively promote dendritic development in these cells [as described in Withers et al., (2000) PMID: 10651865]. The coverslip containing neurons was mounted in a sealed temperature controlled chamber (Warner Instruments) in phenol-free Minimal Essential Medium supplemented with N2, pyruvate, and 30 ng/ml human recombinant BMP-7. Recording was made using an inverted Leica DMIRBE microscope, 40X PL Fluotar, 0.75 N.A. objective, Princeton Instruments MicroMax camera, and MetaMorph software. Images were acquired every 5 minutes, beginning 5 hours after plating, and continuing for over 19 hours. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Withers, Ginger (2021). CIL:701, Rattus, multipolar neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J07081GB
Type
image
Identifier
ark:/20775/bb2089451w
Language
No linguistic content
Subject
Dendrite morphogenesis Dendrite development Establishment or maintenance of cell polarity Dendrite Multipolar neuron Axon Dendritic branch Dendritic growth cone Axonal growth cone Rattus
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
