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Description
Time-lapse of Toxoplasma gondii. A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgIMC1 and mRFP-tagged TgMORN1 (MORN domain containing protein). Formation of daughter cells is visible during time-lapse. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: EGFP, ex 450-490 em 500-540, mRFP, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. Stacks of images were collected at 0.6 μ increments in z, every 10 min. This 3D deconvolved data corresponds with raw data in CIL# 10522. Projection of deconvolved stack: CIL#10524 and movie of projection: 10525. Unpublished image that is similar to images published in PMID: 16518471 and 20140195. See publications for other experimental details. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Murray, John; Hu, Ke (2021). CIL:10523, Toxoplasma gondii RH. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J07W6BBG
Type
image
Identifier
ark:/20775/bb22597309
Language
No linguistic content
Subject
Regulation of cell shape Cytoskeleton organization Cell division Toxoplasmosis Toxoplasma gondii RH stable cell line Spindle pole Inner membrane complex Conoid Basal part of cell Centriole Toxoplasma gondii RH Cell Image Library Group ID: 5774
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