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Description
NG108-15 cells expressing EGFP-actin. NG108-15 cells are a cell line that is derived from a mouse neuroblastoma and a rat glioma. This is one of two images related images that show actin dynamics in the growth cones of the NG108 cells. This image shows a more detailed view of filopodial dynamics in the growth cone and the related image shows captures the actin dynamics of multiple processes on the NG108. Images were collected as a single z slice time series on a Carl Zeiss 5 LIVE microscope. The Zeiss 5 LIVE uses a cylindrical lense to create a sheet of illumination that is rapidly scanned in the vertical direction and detected on a linear CCD array to enable faster imaging. The images were collected with a 63X 1.4 NA plan apo objective. Filers: NFT 490 and BP 500-525. 17 micron slit width. Pixel dwell 1.94 ms yielded approximately 2 frames collected per s. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Galbraith, Catherine G.; Galbraith, James A. (2021). CIL:7249, mouse/rat NG108-15. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0H70DT7
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