Bennetzen, Martin v Bénit, Paule Cabrera, Sandra Criollo, Alfredo Eisenberg, Tobias Galluzzi, Lorenzo Horio, Yoshiyuki Kepp, Oliver Maiuri, Maria Chiara Malik, Shoaib Ahmad Mariño, Guillermo Megalou, Evgenia Morselli, Eugenia Rustin, Pierre Schroeder, Sabrina Shen, Shensi
Contributor
Bennetzen, Martin v. Bénit, Paule Cabrera, Sandra Criollo, Alfredo Eisenberg, Tobias Galluzzi, Lorenzo Horio, Yoshiyuki Kepp, Oliver Maiuri, Maria Chiara Malik, Shoaib Ahmad Mariño, Guillermo Megalou, Evgenia Morselli, Eugenia Rustin, Pierre Schroeder, Sabrina Shen, Shensi
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Description
Image indicative of 24 h autophagic activity of human colon carcinoma HCT 116 cell co-transfected with a RFP-LC3-encoding plasmid and a SIRT1 variant with a mutation in the nuclear localization signal fused to GFP (mtNLS GFTP-SIRT1) which is virtually restricted to the cytoplasm. Confocal fluorescent images were captured using a confocal fluorescence microscope (TCS SP2; Leica) fitted with an Apochromat 63× 1.3 NA immersion objective. Images were acquired with a camera (DFC 350 FX 1.8.0; Leica) using LAS AF software (Leica) and processed with Photoshop (CS2; Adobe) software. Specifically, picture processing involved cropping of representative areas and linear adjustments of contrast and brightness and was performed using Photoshop (with equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 8C, bottom panel, in Morselli et al. J Cell Biol 192: 615-629 Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Morselli, Eugenia; Mariño, Guillermo; Bennetzen, Martin v.; Eisenberg, Tobias; Megalou, Evgenia; Schroeder, Sabrina; Cabrera, Sandra; Bénit, Paule; Rustin, Pierre; Criollo, Alfredo; Kepp, Oliver; Galluzzi, Lorenzo; Shen, Shensi; Malik, Shoaib Ahmad; Maiuri, Maria Chiara; Horio, Yoshiyuki (2021). CIL:13914, Homo sapiens, permanent cell line cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0HH6HS2
Type
image
Identifier
ark:/20775/bb2361605d
Language
No linguistic content
Subject
NAD-dependent protein deacetylase activity Autophagy HCT 116 Permanent cell line cell Autophagic vacuole Cytoplasm Homo sapiens Cell Image Library Group ID: 5408
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