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Image / CIL:37378, damselfly, flight muscle cell

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Title
CIL:37378, damselfly, flight muscle cell
Creator
Franzini-Armstrong, Clara
Loesser, Kathryn
Contributor
Franzini-Armstrong, Clara
Loesser, Kathryn
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Creative Commons Public Domain Dedication
Constraint(s) on Use: This work may be used without prior permission.
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description
Longitudinal and cross sections showing ribbon like myofibrils regularly alternating with layers of mitochondria and SR/T tubules. Dyads are located in a grove of the mitochondrial surface. Negatives aere scanned on nikon 8000 at 4000dpi/6.3 micron scan step size, then reduced in size 4X for upload, for an effective pixel size of 25 micron. Technical details: Heads and tail removed, thorax bisected and immersed in 3% glutaraldehyde buffered in 0.1 M cacodylate buffer (pH7.2). Stored at 4C for several days. Muscle dissected out. Rinsed 3Xin buffer, postfixed in 2% PsO4 in buffer, for 1.5 hrs; rinsed 3 times in water, en-block stained in saturated aqueous uranyl acetate, embedded in Spurr. Thin sections stained in uranyl acetate and lead and examined in a Philips 410 Microscope. Reference for the image: Loesser, K.E., Castellani, L. and Franzini-Armstrong, C. Disposition of junctional feet in muscles of invertebrates. J. Muscle Research Cell Motility 13:161-173, 199
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Franzini-Armstrong, Clara; Loesser, Kathryn (2021). CIL:37378, damselfly, flight muscle cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09S1QBQ
Type
image
Identifier
ark:/20775/bb2464704x
Language
No linguistic content
Subject
Sequestering of calcium ion
Membrane depolarization
Mitochondrial proton-transporting ATP synthase complex assembly
Skeletal muscle contraction
Flight muscle cell
Sarcoplasmic reticulum
Myofibril
Mitochondrion
T-tubule
Damselfly

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