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Image / CIL:36637, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist

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Title
CIL:36637, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
Creator
Allen, Richard
Contributor
Allen, Richard
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Creative Commons Public Domain Dedication
Constraint(s) on Use: This work may be used without prior permission.
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description
Like other cells Paramecium has peroxisomes. These organelles are usually smaller than mitochondria and have a granular appearance and contain a few tubular-like inclusions. These tubules have not been conclusively shown to be continuous with the single limiting membrane of this organelle. In this case the peroxisome has a microtubule lying along its surface. This cell was exposed to HRP for 30 seconds and then chased in HRP-free medium for 3 minutes. The subsequent incubating with hydrogen peroxidase and diaminobenzidine resulted in an electron opaque reaction product in the peroxisomes not because they took in HRP but because the peroxisomes contain large amounts of catalase that results in the reduction of hydrogen peroxidase. HRP can only enter the living cell by endocytosis, phagocytosis, when trichocysts are discharged, at the open cytoproct or when a CV opens. TEM taken on 6/13/79 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 22,500X. Bar = 0.25µm.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Allen, Richard (2021). CIL:36637, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0R49PQR
Type
image
Identifier
ark:/20775/bb29424832
Language
No linguistic content
Subject
Organelle organization
Cytoplasm organization
Peroxisome organization
Eukaryotic Protist
Eukaryotic cell
Ciliated Protist
Cell by organism
Cytoplasm
Peroxisome
Paramecium multimicronucleatum

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