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Description
A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing YFP-tagged TgIMC1 (innermembrane complex scaffold protein) and mCherry-tagged human alpha1tubulin. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: mCherry, ex 555-592 em 600-665, YFP, ex 490-510 em 525-595. Captured with CoolSnapHQ/ICX285. This is a maximum intensity projection from z slices 3-6 from deconvolved data stack: CIL# 10539. Maximum intensity projection from z slices 10-13: CIL# 10540. Corresponding raw data stack: CIL# 10538 and raw data stack with DIC: CIL# 10543. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Murray, John; Hu, Ke (2021). CIL:10541, Toxoplasma gondii RH. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02R3QSS
Type
image
Identifier
ark:/20775/bb33518900
Language
No linguistic content
Subject
Microtubule cytoskeleton organization Structural constituent of cytoskeleton Toxoplasmosis Toxoplasma gondii RH stable cell line Regulation of cell shape Cell division Conoid Cortical microtubule Centriole Inner membrane complex Spindle pole Toxoplasma gondii RH Cell Image Library Group ID: 5765
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