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Description
Visualization of clathrin coated pit dynamics. Total internal reflection fluorescencec microscopy (TIRF-FM) was performed on live human retinal pigment epithelial (RPE) cells stably expressing 'clathrin light chain a' tagged with EGFP (EGFP-LCa) using a 100x 1.45 NA objective (Nikon) mounted on a Nikon TE2000U inverted microscope (Nikon) coupled to a 14-bit mode operated Hamamatsu Orca II-ERG camera. This single channel TIR-FM movie, showing clathrin coated pit dynamics at the ventral plasma membrane, was acquired at a frame rate of 1s with a 70 msec exposure time during 10 min. Pixel size: 67 nm Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Mettlen, Marcel; Schmid, Sandra L. (2021). CIL:10136, Homo sapiens, epithelial cell, human retinal pigmented epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0DN4454
Type
image
Identifier
ark:/20775/bb3454268k
Language
No linguistic content
Subject
Clathrin coated pit dynamics Epithelial cell Clathrin light chain a Clathrin coat of coated pit Human retinal pigmented epithelial cell Homo sapiens
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