Under copyright Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work. Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
A population of CHO cells constitutively overexpressing a clone of human IgG2/kappa. CHO cells are regarded as the workhorse of recombinant protein expression and biopharmaceutical production. Under certain conditions such as when protein synthesis and protein folding efficiency exceed the capacity of ER-to-Golgi transport, export ready IgG species can progressively accumulate in the ER until a phase separation threshold of the protein is reached to crystallize. This human IgG2/kappa clone induced rod-shaped crystalline body in the ER of engineered CHO cells. Protein crystallization events physically prevented cytokinesis, without hampering nuclear division. The net result was the multi-nucleation and marked cell enlargement. The size of crystal-laden cells became markedly larger than the cells that do not house crystals. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Hasegawa, Haruki (2021). CIL:48061, Cricetulus griseus, Ovary cells, CHO cells. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0833S69
Type
image
Identifier
ark:/20775/bb35574064
Language
No linguistic content
Subject
Endoplasmic reticulum organization Cytoplasm organization Endoplasmic reticulum CHO cells Crystalline body Ovary cells Inclusion body Crystalloid Cricetulus griseus Cell Image Library Group ID: 17454
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
