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Image / CIL:11837, Potorous tridactylus, epithelial cell

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Title
CIL:11837, Potorous tridactylus, epithelial cell
Creator
Bokoch, Gary M
Waterman-Storer, Clare M
Wittmann, Torsten
Contributor
Bokoch, Gary M.
Waterman-Storer, Clare M.
Wittmann, Torsten
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
Microtubules in a dominant-negative Rac1(T17N)-expressing PtK1 cell. Microtubules do not undergo retrograde flow, and microtubules rapidly switch between growth and shortening phases with little net change in length over time. The cell was microinjected with X-rhodamine-conjugated tubulin and an EGFP-Rac1(T17N) expression plasmid, and was observed by fluorescence microscopy. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 3 of associated paper (J Cell Biol, 161:845-851, 2003).
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Wittmann, Torsten; Bokoch, Gary M.; Waterman-Storer, Clare M. (2021). CIL:11837, Potorous tridactylus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00Z722G
Type
image
Identifier
ark:/20775/bb37267362
Language
No linguistic content
Subject
Microtubule polymerization
Dominant-negative Rac
Pt K1 (NBL-3)
Epithelial cell
Microtubule
Potorous tridactylus
Cell Image Library Group ID: 4105

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