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Description
Localization of cross-linking proteins in fibroblast cytoskeleton. Fluorescence microscopy and corresponding intensity profiles of Xenopusfibroblast lamellipodia double stained with TRITC-phalloidin (red) and either p21 (Arp 2/3) antibodies (green). The protein/actin ratio at the leading edge of the lamellipodium is high for Arp2/3 complex. Immuno-EM of the cell edge (CIL 34925) or interior (CIL 34928) of cytochalasin D-treated Xenopus fibroblasts stained with p21 primary antibody and 10-nm gold-conjugated secondary antibody reveal Arp2/3 complex at Y-junctions. Image corresponds to Figure 5a and a' from J Cell Biol. 1999 May 31;145(5):1009-26. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Svitkina, Tatyana M.; Borisy, Gary G. (2021). CIL:34922, Xenopus laevis, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09K48WK
Type
image
Identifier
ark:/20775/bb3726915g
Language
No linguistic content
Subject
Cellular localization Arp2/3 protein complex Lamellipodium Actin cytoskeleton Fibroblast Xenopus laevis Cell Image Library Group ID: 9875
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