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Description
QuickTime movie of a complete optical scan through a rabbit intestinal villus tip infected with Wt-GFP L. monocytogenes (green), and counterstained with phalloidin (red) to visualize the actin cytoskeleton, and with toto-3 (blue) to visualize nuclei. Paraformaldehyde fixed intact tissue blocks were processed without prior embedding and sectioning. The samples were imaged by confocal microscopy where optical sections were taken at 0.5 micron resolution through both the cell monolayers and the intestinal villi. Z-stacks were reconstructed into three dimensions using Volocity software. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Pentecost, M; Otto, G.; Theriot, J.A.; Amieva, M.R. (2021). CIL:25361, Oryctolagus cuniculus, Listeria monocytogenes. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0F47N1T
Type
image
Identifier
ark:/20775/bb3760961v
Language
No linguistic content
Subject
Bacterial invasion at epithelial junctions Nucleus Actin filament Oryctolagus cuniculus Listeria monocytogenes Cell Image Library Group ID: 6802
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