Under copyright Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work. Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
Cultured growth cone (NG108) labeled for F-actin with phalloidin (red) and conformationally activated beta1 integrin adhesion receptors with 9EG7 antibody (green). The adhesion molecules at the tips of the growth cone filopodia are primed to bind the extracellular matrix. These "sticky fingers" appear to search for sites to form adhesions that can be used to move the rest of the cell forward. Collected on Zeiss LSM 510 with a 63X plan Apo 1.4 NA objective. Pinholes adjusted around 1 Airy Unit to yield match volume according to wavelength. 488 actin image BP: 505-530, 532 activated integrin image: LP:560. Image format: red/green maximum projection followed by actin z-series and activated beta1 integrin z series. Related to Figure 4B in Science. 2007, 315(5814):992-5. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Galbraith, Catherine G.; Galbraith, James A. (2021). CIL:131, mouse/rat NG108-15, glioma, neuroblastoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09C6W4V
Type
image
Identifier
ark:/20775/bb3897381h
Language
No linguistic content
Subject
Cell adhesion mediated by integrin Actin polymerization-dependent cell motility involved in migration of symbiont in host NG108-15 Extracellular matrix binding Structural constituent of cytoskeleton Growth cone Neuroblastoma Filopodium Glioma Mouse/rat NG108-15
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
