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Description
The events of meiosis I and II in a living insect spermatocyte beginning with the breakdown of the nuclear envelope at diakinesis through to the completion of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 2 min over 5 hr 18 min by a QImaging Retiga EXi CCD camera. Raw images were processed using a 5-frame algorithm (Shribak and Oldenbourg, 2003). Movie images represent computed retardance as gray values ranging from 0 nm retardance (black) to 2 nm retardance or higher (white). Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Oldenbourg, Rudolf; LaFountain, James R. (2021). CIL:9060, Nephrotoma suturalis, spermatocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0RN36W3
Type
moving image
Identifier
ark:/20775/bb4307482n
Language
No linguistic content
Subject
Male meiosis chromosome segregation Spindle assembly involved in male meiosis Nuclear chromosome Cytoskeleton Spindle Spermatocyte Nephrotoma suturalis Cell Image Library Group ID: 3782
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