Image / CIL:13124, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist

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Title: CIL:13124, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
Creator: Allen, Richard
Contributor: Allen, Richard
Date Created and/or Issued: 2021
Contributing Institution: UC San Diego, Research Data Curation Program
Collection: Cell Image Library
Rights Information: Creative Commons Public Domain Dedication
Constraint(s) on Use: This work may be used without prior permission.
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description: A high resolution micrograph of the collecting canal of the contractile vacuole surrounded by smooth spongiome remnants and decorated tubules of the CVC. Microtubules bordering the flattened collecting canal are labeled with immunogold when thin frozen sections of the CVC are exposed by the indirect immunogold technique to show anti-b-tubulin. These microtubules have their origin at the cytosolic side of the CV pore. TEM taken on 2/25/94 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are outlined in Methods Cell Biol. 2010;96:143-73. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Allen, Richard (2021). CIL:13124, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0M044KT
Type: image
Identifier: ark:/20775/bb43075645
Language: No linguistic content
Subject: Contractile vacuole organization
Contractile vacuolar membrane
Eukaryotic cell
Cell by organism
Cytoplasmic microtubule
Eukaryotic Protist
Contractile vacuole
Ciliated Protist
Paramecium multimicronucleatum
Cell Image Library Group ID: 9800

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