Image / CIL:13093, Acanthamoeba castellanii, cell by organism, eukaryotic cell, amoeboid cell, Eukaryotic Protist

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Title: CIL:13093, Acanthamoeba castellanii, cell by organism, eukaryotic cell, amoeboid cell, Eukaryotic Protist
Creator: Kaiser, Don
Contributor: Kaiser, Don
Date Created and/or Issued: 2021
Contributing Institution: UC San Diego, Research Data Curation Program
Collection: Cell Image Library
Rights Information: Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact: UC Regents
Description: To characterize and validate the gluteraldehyde fixation method, a live amoeba syringe-loaded with Rhodamine-N58C-profilin-II was monitored in a flow cell as the fixative was introduced. This time series of cooled CCD fluorescence micrographs shows a cell as it is being fixed in 20% Dulbecco's PBS at pH 6.5 containing 1% glutaraldehyde at room temperature. See publication below for details. Live cells were observed with a Zeiss Axiovert 135 fluorescence microscope. Electronic imaging of fluorescent specimens was performed using a Photometrics cooled, charge-coupled device (CCD) camera (PXL-1400) controlled with IPLab Spectrum 3.1P software (Scanolytics, Fairfax, VA) on Macintosh computers. Copyright © 1999 by Company of Biologists and reproduced/adapted with permission in 2011.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Kaiser, Don (2021). CIL:13093, Acanthamoeba castellanii, cell by organism, eukaryotic cell, amoeboid cell, Eukaryotic Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0CN72ND
Type: image
Identifier: ark:/20775/bb4545888x
Language: No linguistic content
Subject: Regulation of lamellipodium assembly
Lamellipodium assembly involved in ameboidal cell migration
Endocytosis
Lamellipodium
Cell by organism
Eukaryotic Protist
Cytoplasm
Endosome
Eukaryotic cell
Amoeboid cell
Acanthamoeba castellanii
Cell Image Library Group ID: 3928

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