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Description
Aggregates of Maize mosaic virus (MMV, Rhabdoviridae) in cells of the salivary gland of the insect vector Peregrinus maidis (planthopper, Hemiptera, Delphacidae). Antibodies to MMV identify aggregates (green/yellow fluorescence). In the salivary gland, MMV aggregates are found mainly in the cell periphery or intercellularly, in contrast to other tissues in the insect vector that accumulate mainly around the nuclei (red). Accompanying image in this group contains labels. Methods: Dissected insect organs were fixed in paraformaldehyde, labeled with primary antibodies to MMV, and with secondary antibodies: Alexa Fluor-488 (green), and later stained with the nuclear stain propidium iodide (red). Whole mounts were examined with confocal laser- scanning microscope (Leica TCS SP). Associated references: Ammar, E.-D. and S. A. Hogenhout, S.A. (2008). A neurotropic route for Maize mosaic virus (Rhabdoviridae) in its planthopper vector Peregrinus maidis. Virus Research 131: 77-85. Ammar, E.-D. and Nault, L.R. (1985). Assembly and accumulation sites of Maize mosaic virus in its planthopper vector. Intervirology 24: 33-41. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Ammar, El-Desouky; Hogenhout, Saskia A.; Nault, L.R. (2021). CIL:25368, Maize mosaic virus, Peregrinus maidis, secretory cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0NS0SN9
Type
image
Identifier
ark:/20775/bb48872522
Language
No linguistic content
Subject
Viral transmission by vector Cell periphery Nucleus Secretory cell Peregrinus maidis Maize mosaic virus Cell Image Library Group ID: 8078
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