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Image / CIL:7240, Homo sapiens, epithelial cell

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Title
CIL:7240, Homo sapiens, epithelial cell
Creator
Hu, Chi-Kuo
Contributor
Hu, Chi-Kuo
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Creative Commons Public Domain Dedication
Constraint(s) on Use: This work may be used without prior permission.
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description
HeLa cell was fixed by MeOH and stained with microtubules (green) and central spindlin protein MKLP1 (red). MKLP1 (mitotic kinesin-like protein) was well aligned on midzone microtubules during cytokinesis. Cells were fixed with MeOH on ice for 3 min, and stained with a primary antibody against MLKP1 and FITC labeled DM1a antibody against microtubules. Secondary antibody was Alexa 594 for MLKP1. A single section was collected with a spinning disk microscope on a Nikon TE-2000 with a 1.3 NA 100X objective. Images were collected wti an OrCA ER CCD camera. Lasers and filters: Innova 70C Spectrum 3 watt Laser, 488 Laser/ATOF 525/50, and 568 Laser/ATOF 605/52.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Hu, Chi-Kuo (2021). CIL:7240, Homo sapiens, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0222SSB
Type
image
Identifier
ark:/20775/bb4955899p
Language
No linguistic content
Subject
Microtubule-based movement
HeLa
Microtubule
Epithelial cell
Homo sapiens
Cell Image Library Group ID: 1455

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