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Image / CIL:25394, Drosophila melanogaster, Salivary gland

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Title
CIL:25394, Drosophila melanogaster, Salivary gland
Creator
Ris, Hans
Contributor
Ris, Hans
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Creative Commons Public Domain Dedication
Constraint(s) on Use: This work may be used without prior permission.
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description
Salivary glands from 3rd instar larvae of Drosophila melanogaster were dissected, fixed with formalin-acetic acid containing 0.1% Triton-X 100, and squashed on a glass slide. After freezing in liquid nitrogen, the cover slips removed, and the slide thawed and stained with 1% uranyl acetate, rinsed in 50% ethanol, dehydrated and transferred to amyl acetate, and covered with 2% parlodion in amyl acetate. Selected areas of the parlodion film, are transferred to formvar coated EM grids, and the parlodion removed by immersion in amyl acetate (see Ris H, 1981 Stereoscopic Electron Microscopy of Chromosomes, Meth Cell Biol 22:77-96). Grids were observed using the Madison High Voltage Electron Microscope at 1 MeV. Image shows a series of bands and interbands in a polytene chromosome. This image was taken with a tilt angle of 45 degrees. The accompanying grouped image, taken at 55 degrees forms a stereo pair, providing an oblique 3D view of the chromosome.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Ris, Hans (2021). CIL:25394, Drosophila melanogaster, Salivary gland. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0S46QWK
Type
image
Identifier
ark:/20775/bb49902051
Language
No linguistic content
Subject
Polytene chromosome
Salivary gland
Drosophila melanogaster
Cell Image Library Group ID: 6839

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