Image / CIL:36735, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist

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Title: CIL:36735, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
Creator: Allen, Richard
Contributor: Allen, Richard
Date Created and/or Issued: 2021
Contributing Institution: UC San Diego, Research Data Curation Program
Collection: Cell Image Library
Rights Information: Creative Commons Public Domain Dedication
Constraint(s) on Use: This work may be used without prior permission.
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description: Cytochalasin B blocks DV release from cytopharynx. In this micrograph a very large nascent vacuole has been retained at the cytopharynx of this cell that had been exposed to cytochalasin B. Many acidosomes are docked at the membrane of this vacuole. Some acidosomes contain HRP reaction product and some are free of HRP. The cell had been exposed to HRP and cytochalasin B for 10 minutes during which no new DV-I had been released. During this exposure to CB, some discoidal vesicles continue to move to the cytopharynx and fuse, acidosomes continue to move to the nascent phagosome and dock, and endosome and carrier vesicle formation continues and they continue to fuse with acidosomes. CB has done two things, it has prevented normal phagosome release from the cytopharynx and it has also inhibited acidosome fusion with the phagosomes (Allen and Fok, J. Cell Biol. 97:566-570, 1983) once they form. For this study cells were exposed to 0.3mM of CB for 10-20 min. The lack of acidosome fusion with the few phagosomes that do pinch off during CB exposure was supported by the fact that the phagosomes became only mildly acid. TEM taken on 8/29/84 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 1,610X. Bar = 2µm.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Allen, Richard (2021). CIL:36735, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0ZW1JV9
Type: image
Identifier: ark:/20775/bb5468111r
Language: No linguistic content
Subject: Response to drug
Digestive system process
Lysosome
Ciliated Protist
Eukaryotic cell
Eukaryotic Protist
Food vacuole
Cell by organism
Paramecium multimicronucleatum

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