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Description
CFP-expressing neuron, 14 days in vitro. By two weeks, cultured hippocampal neurons have developed a morphology typical of mature neurons with an extensive axonal arbor, a tapered and branched dendritic arbor, and synaptic connections. Growth cones can be seen at the tips of axonal branches, and the dendritic arbor is studded with spines, specialized structures commonly associated with excitatory synapses. This image accompanies the second file in this image group combines the fluorescent and phase micrographs from the same field of view. Embryonic rat hippocampal neurons were prepared and transfected with soluble CFP (excitation, 439; emission, 476) at plating (Dotap, Roche) as previously described (Kaech and Banker, 2006, Nat Protoc), fixed for 15 minutes (4% formaldehyde, 4% sucrose in phosphate buffered saline, pH 7.4, warmed to 37°C, permeabilized with 0.25% Triton X-100 for 7 minutes, and rinsed with phosphate buffered saline at 14 days in vitro (Withers and Banker, 1998, in Culturing Nerve Cells, MIT Press). Images were acquired with a Leica DMRXA microscope with a mercury arc lamp, a 16X PL Fluotar (0.5 NA) oil immersion lens, Princeton Instruments Micromax CCD camera and MetaMorph software, using a Leica CFP filter cube. Linked images show: A) CFP expression throughout the extent of the neuron; B) a merged image of the fluorescent cell with respect to the phase image, generated with the MetaMorph overlay function. The single plane fluorescence image is included in this image group. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Withers, Ginger (2021). CIL:846, Rattus, multipolar neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0542NNH
Type
image
Identifier
ark:/20775/bb54683256
Language
No linguistic content
Subject
Establishment or maintenance of cell polarity Dendrite development Cellular developmental process Multipolar neuron Dendrite Neuron spine Growth cone Axon Rattus Cell Image Library Group ID: 7904
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