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Description
Single computed slice through a double tilt electron tomogram of a lamellar structure observed in HeLa cells transfected with mutant connexin 50, imaged using intermediate voltage electron microscopy, and labeled using the tetracysteine-ReHAsH system and fluorescence photoooxidation. The concentric double membrane layers of the aggregate appear to form "onion skin" sheets that do not form a closed structure along the long axis. The differential staining between the outside and inside is most likely due to impeded diffusion of DAB into the interior of these accumulations. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For more information see: Lichtenstein et al. The cytoplasmic accumulations of the cataract-associated mutant, Connexin50P88S, are long-lived and form in the endoplasmic reticulum Exp Eye Res. 2009 Mar;88(3):600-9. PMID: 19073179 Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Beyer, Eric (2021). CIL:40187, Homo sapiens. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0T43T61
Type
image
Identifier
ark:/20775/bb5502798h
Language
No linguistic content
Subject
HeLa Connexon complex Plasma membrane Homo sapiens Cell Image Library Group ID: 12153
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