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Description
Time-lapse of a CHO.K1 cell cotransfected with very low levels of GFP-MIIA (myosin IIA, green) and paxillin (magenta). Yellow arrowheads mark paxillin-containing adhesions that disassemble concomitantly to their MIIA-decorated bundle (marked with arrows) as the movie progresses. Total internal reflection fluorescence (TIRF) images were acquired on an inverted microscope (IX70; Olympus) with a 1.45 NA (oil) Plan-Apochromat 60× TIRFM objective lens. Images were acquired with a charge-coupled device (CCD) camera (Retiga Exi; Qimaging). Video corresponds to Fig 5C and Video 5 from J Cell Biol 2011 193:381-396. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Vicente-Manzanares, Miguel; Newell-Litwa, Karen; Bachir, Alexia I.; Whitmore, Leanna A.; Horwitz, Alan Rick (2021). CIL:25846, Cricetulus griseus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0X9295N
Type
image
Identifier
ark:/20775/bb5569857p
Language
No linguistic content
Subject
CHO-K1 Focal adhesion disassembly Epithelial cell Focal adhesion Myosin II complex Cricetulus griseus Cell Image Library Group ID: 7724
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