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Title
CIL:39733
Creator
Fairall, Louise
Huynh, Van A
Rhodes, Daniela
Robinson, Philip J
Contributor
Fairall, Louise
Huynh, Van A.
Rhodes, Daniela
Robinson, Philip J.
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
EM images of chromatin fibers reconstituted from tandem arrays of a strong nucleosome positioning sequence flanked by 'linker' DNA, creating fibers containing 22 nucleosomes and a nucleosome repeat length (NRL) of 177 bp. Reconstitutes had saturating amounts of purified core histones and linker histones. (A) shows a montage of 'end on' views of fibers obtained by cryo-fixation and recorded in the frozen hydrated state. (B and C) contain views of 3D models considered to correspond to the images at left. See Fig 1 in P.J. Robinson et al. 2006. EM measurements define the dimensions of the "30-nm" chromatin fiber: Evidence for a compact, interdigitated structure. Proc Natl Acad Sci USA 103:6506-6511.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Robinson, Philip J.; Fairall, Louise; Huynh, Van A.; Rhodes, Daniela (2021). CIL:39733. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0416VVV
Type
image
Identifier
ark:/20775/bb57065120
Language
No linguistic content
Subject
Chromatin organization
DNA organization
Chromatin
Cell Image Library Group ID: 10718

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