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Description
To study the cellular consequences of misfolding of plasma membrane proteins, a transmembrane model protein that was constitutively targeted to the plasma membrane was developed for use as a reporter molecule. The reporter consisted of a C-terminally truncated CD4, incorporating a flexible cytoplasmic linker (CD4tl), fused to the N-terminal DNA-binding domain of the wild type (wt) bacteriophage lambda repressor (CD4tl-lambda) or L57C mutant repressor (CD4tl-lambdaC). The CD4tl-lambdaC cytosolic domain was largely in native state at 26°C but predominantly nonnative state at 37°C thus allowing the use of thermal shifts to follow the fate of unfolded proteins. In this image, one of six of a group from Figure 1 of Pirjo et al., JCB 2010, the expression of the control reporter, CD4tl-lambda, at 37 degrees is seen with CD4 antibody (green) in a detergent-permeabilized COS7 cell and the apparent plasma membrane targeting shows the value of this model plasma membrane protein. The cell is co-labeled for an ER marker, calreticulin antibody (red).. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Apaja, Pirjo M.; Xu, Haijin; Lukacs, Gergely L. (2021). CIL:13684, Chlorocebus aethiops. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0GT5KWN
Type
image
Identifier
ark:/20775/bb57404620
Language
No linguistic content
Subject
Protein targeting to plasma membrane COS-7 Endoplasmic reticulum Integral to plasma membrane Chlorocebus aethiops Cell Image Library Group ID: 7831
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