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Description
Aggregates of maize mosaic virus (MMV, Rhabdoviridae) in cells of the anterior diverticulum of the insect vector Peregrinus maidis (planthopper, Hemiptera, Delphacidae). Antibodies to MMV identify aggregates (green/yellow fluorescence) that accumulate mainly around the nuclei (red) in cells of the anterior diverticulum. Accompanying image in this group contains a scale bar. Methods: Dissected insect organs were fixed in paraformaldehyde, labeled with primary antibodies to MMV, and with secondary antibodies: Alexa Fluor-488 (green), and later stained with the nuclear stain propidium iodide (red). Whole mounts were examined with confocal laser- scanning microscope (Leica TCS SP). Associated references: Ammar, E.-D. and S. A. Hogenhout, S.A. (2008). A neurotropic route for Maize mosaic virus (Rhabdoviridae) in its planthopper vector Peregrinus maidis. Virus Research 131: 77-85. Ammar, E.-D. and Nault, L.R. (1985). Assembly and accumulation sites of Maize mosaic virus in its planthopper vector. Intervirology 24: 33-41. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Ammar, El-Desouky; Hogenhout, Saskia A.; Nault, L.R. (2021). CIL:25380, Maize mosaic virus, Peregrinus maidis. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0N0159T
Type
image
Identifier
ark:/20775/bb5774635s
Language
No linguistic content
Subject
Viral transmission by vector Host cell perinuclear region of cytoplasm Nucleus Peregrinus maidis Maize mosaic virus Cell Image Library Group ID: 8075
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