Creative Commons Public Domain Dedication Constraint(s) on Use: This work may be used without prior permission. Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Description
This is IMAGE #11 (second time-lapse set, 50 minutes) of a time lapse series contained with in this image group. It shows neurons from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47. The images were acquired with ~10 minute intervals and show morphological plasticity of the dendritic arbor with branch additions and retractions. These cells were transfected ~24 hours earlier with in vivo electroporation of plasmid DNA encoding eYFP. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope. Images were acquired a 0, 10, 20, 30, and 50 minutes. After 60 minutes, the animal was placed in a 50 microMolar anisomycin solution for ~4 hours. A second time lapse series (10 minute interval for 60 minutes) was then acquired. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Bestman, Jennifer; Cline, Holly (2022). CIL:36893, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0FT8KV1
Type
image
Identifier
ark:/20775/bb6457887w
Language
No linguistic content
Subject
Neuron development Translation Morphogenesis of a branching structure Neuronal cell body Dendrite Cytoplasm CNS neuron (sensu Vertebrata) Xenopus laevis Cell Image Library Group ID: 9194
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
