Bennetzen, Martin v Bénit, Paule Cabrera, Sandra Criollo, Alfredo Eisenberg, Tobias Galluzzi, Lorenzo Horio, Yoshiyuki Kepp, Oliver Maiuri, Maria Chiara Malik, Shoaib Ahmad Mariño, Guillermo Megalou, Evgenia Morselli, Eugenia Rustin, Pierre Schroeder, Sabrina Shen, Shensi
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Bennetzen, Martin v. Bénit, Paule Cabrera, Sandra Criollo, Alfredo Eisenberg, Tobias Galluzzi, Lorenzo Horio, Yoshiyuki Kepp, Oliver Maiuri, Maria Chiara Malik, Shoaib Ahmad Mariño, Guillermo Megalou, Evgenia Morselli, Eugenia Rustin, Pierre Schroeder, Sabrina Shen, Shensi
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Transgenic C57BL/6 mice expressing GFP-LC3 fusion protein, uninjected as control for injection with Resveratrol and/or spermidine. To avoid postmortem autophagy induction, dead mice were immediately perfused with 4% paraformaldehyde (wt/vol in PBS, pH 7.4). Tissues were then harvested and further fixed with the same solution for ≥4 h followed by treatment with 15% sucrose (wt/vol in PBS) for 4 h and with 30% sucrose (wt/vol in PBS) overnight. Tissue samples were embedded in Tissue-Tek OCT compound (Sakura) and stored at −70C. 5-µm-thick tissue sections were generated with a cryostat (CM3050 S; Leica), air dried for 1 h, washed in PBS for 5 min, dried at RT for 30 min, and mounted with Vectashield antifading medium. Confocal fluorescent images were captured using a confocal fluorescence microscope (TCS SP2; Leica) fitted with an Apochromat 40× 1.15 NA immersion objective. Images were acquired with a camera (DFC 350 FX 1.8.0; Leica) using LAS AF software (Leica) and processed with Photoshop (CS2; Adobe) software. Specifically, picture processing involved cropping of representative areas and linear adjustments of contrast and brightness and was performed using Photoshop (with equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 9C, middle left panel (muscle/Co), in Morselli et al. J Cell Biol 192: 615-629 Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Morselli, Eugenia; Mariño, Guillermo; Bennetzen, Martin v.; Eisenberg, Tobias; Megalou, Evgenia; Schroeder, Sabrina; Cabrera, Sandra; Bénit, Paule; Rustin, Pierre; Criollo, Alfredo; Kepp, Oliver; Galluzzi, Lorenzo; Shen, Shensi; Malik, Shoaib Ahmad; Maiuri, Maria Chiara; Horio, Yoshiyuki (2021). CIL:13925, Mus musculus, skeletal muscle cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J04Q7SQH
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