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Description
HeLa cells at last stage of cytokinesis were fixed and stained with microtubules (green), PRC1 (red), and KIF4A (blue). PRC1 and KIF4A colocalized on midbody in the center of intracellular bridge and a dark zone which cannot be accessed by antibodies was shown. Cells were fixed with MeOH on ice for 3 min, and stained with primary antibodies against PRC1 (Protein regulator of cytokinesis 1), KIF4A (Chromosome-associated kinesin), and FITC-DM1a antibody against microtubules. Secondary antibodies were Alexa 594 for PRC1, and Alexa 647 for KIF4A. A single section was collected with a spinning disk microscope on a Nikon TE-2000 with a 1.3 NA 100X objective. Images were collected with an OrCA ER CCD camera. Lasers and filters: Innova 70C Spectrum 3 watt Laser, 488 Laser/ATOF 525/50, 568 Laser/ATOF 605/52, and 647 Laser ATOF 700/75. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Hu, Chi-Kuo (2021). CIL:7244, Homo sapiens, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02R3QN0
Type
image
Identifier
ark:/20775/bb66623995
Language
No linguistic content
Subject
HeLa Microtubule motor activity Cytokinesis Microtubule Midbody Kinesin complex Epithelial cell Homo sapiens Cell Image Library Group ID: 1455
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