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Description
The events of meiosis II in a living insect spermatocyte beginning at the completion of prophase II through the comletion of cytokinesis. Testes from the Crane fly Nephrotoma suturalis were observed with time-lapse polarization using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 1.5x zoom lens. Image acquisition and processing Camera: Images captured every 30 sec over 3 hr by a Dage-MTI C300 video camera with Scion AG-5 frame grabber. Raw images were processed using a 4-frame algorithm (Shribak and Oldenbourg, 2003). Movie images represent computed retardance as gray values ranging from 0 nm retardance (black) to 2 nm retardance or higher (white). Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Oldenbourg, Rudolf; LaFountain, James R. (2021). CIL:9058, Nephrotoma suturalis, spermatocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0NK3D1R
Type
image
Identifier
ark:/20775/bb70037539
Language
No linguistic content
Subject
Spindle assembly involved in male meiosis II Spermatocyte Cytoskeleton Spindle Nuclear chromosome Nephrotoma suturalis Cell Image Library Group ID: 3782
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