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Description
Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP W766G. Endogenous Survivin is green and DAPI (DNA) is blue. See Supplementary Fig 2B panels 9-12 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Xu, Z.; Ogawa, H.; Vagnarelli, P.; Bergmann, J.H.; Hudson, D.F.; Ruchard, S.; Fukagawa, T.; Earnshaw, W.C.; Samejima, K. (2021). CIL:12206, Gallus gallus gallus, memory B cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0K35SDJ
Type
image
Identifier
ark:/20775/bb70373607
Language
No linguistic content
Subject
DT40 Mitosis Chromosome, centromeric region Spindle Nuclear chromosome Memory B cell Gallus gallus gallus Cell Image Library Group ID: 4116
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