Moving Image / CIL:19326, Acanthamoeba castellanii, cell by organism, eukaryotic cell, Eukaryotic Protist, Amoeboid Protist

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Title: CIL:19326, Acanthamoeba castellanii, cell by organism, eukaryotic cell, Eukaryotic Protist, Amoeboid Protist
Creator: Dunnebacke, Thelma
Contributor: Dunnebacke, Thelma
Date Created and/or Issued: 2021
Contributing Institution: UC San Diego, Research Data Curation Program
Collection: Cell Image Library
Rights Information: Creative Commons Public Domain Dedication
Constraint(s) on Use: This work may be used without prior permission.
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description: Acanthamoeba castellani is a free living amoebae found in soil and water and can be an opportunistic pathogen as well. They have been diagnosed as the causative agent associated with keratitis, an infection of the eye, in humans. Other opportunistic pathogens are Naegleria and Balamuthia which have been associated with amebic encephalitis. Acanthamoeba and Naegleria feed by phagocytosis while Balamuthia feed by inserting the tips of their pseudopodia into cells. The other members of the video group, Naegleria and Balamuthia, are linked to this movie. The cells were grown in axenic media. Then they were photographed with a Canon Power Shot G3 with a setting at ISO 50 with an optical microlens at 4X and the quality set at RAW. The camera was attached to the phototube of the microscope with a 3X lens and photographed in phase contrast with a 100X oil immersion objective. Live amoebae were placed in a chamber prepared by inverting a coverslip with/or without cultured mammalian cells onto a 3X1 inch microscope slide with a thin rim of Vaseline; melted paraffin was brushed around the edge of the coverslip to seal it to the slide. Such cultures could be maintained for several days at room temperature.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Dunnebacke, Thelma (2021). CIL:19326, Acanthamoeba castellanii, cell by organism, eukaryotic cell, Eukaryotic Protist, Amoeboid Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0TQ60PC
Type: moving image
Identifier: ark:/20775/bb73792846
Language: No linguistic content
Subject: Pseudopodium assembly
Pseudopodium organization
Pseudopodium retraction
Eukaryotic Protist
Cell
Eukaryotic cell
Pseudopodium
Amoeboid Protist
Cell by organism
Acanthamoeba castellanii
Cell Image Library Group ID: 6503

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