Image / CIL:36721, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist

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Title: CIL:36721, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
Creator: Allen, Richard
Contributor: Allen, Richard
Date Created and/or Issued: 2021
Contributing Institution: UC San Diego, Research Data Curation Program
Collection: Cell Image Library
Rights Information: Creative Commons Public Domain Dedication
Constraint(s) on Use: This work may be used without prior permission.
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description: A mAb, B25-1-3, was raised to antigen Q2 (arbitrary code) to the same set of membranes as mAb 132-F4-G6 labels. This mAb was more reactive on frozen sections. To tell the age of vacuoles, cells were exposed to latex beads for 2.5 minutes and then washed free of the beads (0.5 min) and then chased in bead-free buffer for a known time before fixing. In this image the labeled phagosome is a DV-I as it contained 1.1µm beads that were present in the medium for the 3 minutes before the cells were fixed. Some vesicles in the cytosol are also labeled but there is very little nonspecific label over the general cytosol or the contents of the vacuole. TEM taken 10/24/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Allen, Richard (2021). CIL:36721, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0HD7TJ0
Type: image
Identifier: ark:/20775/bb76865616
Language: No linguistic content
Subject: Digestive system process
Cell by organism
Ciliated Protist
Food vacuole
Eukaryotic Protist
Eukaryotic cell
Pre-autophagosomal structure membrane
Paramecium multimicronucleatum

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