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Description
Polymerized actin localization in wild type Arabidopsis thaliana leaf trichomes, stage 4, during the transition to branch elongation. Detailed methods: Intact Arabidopsis shoots were fixed in 100 mM PIPES/KOH pH 6.9, 5 mM EGTA, 4 mM MgCl2 (PEM) containing 2 % formaldehyde. Actin filaments were detected with overnight incubation with 0.2 µM Alexa Fluor 488® phalloidin. Confocal images were obtained using a Biorad Radiance 2100 confocal head mounted on a Nikon E800 stand, excitation wavelength was the 488 line of a mixed gas Argon ion laser. The objective used was a 60X 1.2 NA water immersion lens, with optical zoom. Acquisition Parameters: Z-stack of confocal images of the actin cytoskeleton, 120 planes with a z-step size of 0.4 µm. Trichomes are unicellular structures that arise from single protodermal cells and usually form three highly elongated branches. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Szymanski, Dan (2021). CIL:14564, Arabidopsis thaliana. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WW7GBD
Type
image
Identifier
ark:/20775/bb8129578v
Language
No linguistic content
Subject
Morphogenesis of a polarized epithelium Positive regulation of Rho GTPase activity Embryonic shoot morphogenesis Arp2/3 protein complex Morphogenesis of a branching structure Leaf trichome development stage Actin cytoskeleton Arabidopsis thaliana Cell Image Library Group ID: 4486
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