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Description
COS 7 cell expressing Organelle LightsTM ER-GFP (Invitrogen) consiting of the signal sequence of the ER protein calreticulin and a KDEL retention sequence. Images were collected with a 63X 1.4 NA Plan-Apochromat oil objective on a Zeiss LSM510 META (Carl Zeiss,Thornwood, NY) using 488 nm excitation, HFT 488 dichroic, and LP505 emission filter. Image size is 1024 x 1024, pixel size is 0.07 µm x 0.07 µm and pixel time was set to 1.28 ¬µsec. Detector pinhole was set to collect 25 ~0.5 µm slices at 0.5 µm intervals. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Patterson, George H. (2021). CIL:721, Chlorocebus sabaeus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0PZ582D
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