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Description
This is IMAGE #4 (day 3) of a time lapse series contained with in this image group. It shows a neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47. The images were acquired daily over 3 days and show dendritic arbor development and morphological plasticity of the dendritic arbor with branch additions and retractions. The superficial layers of the z-stack show the autofluorescence of a pigment cell. This cell was transfected ~24 hours before the first image with in vivo electroporation of plasmid DNA encoding eYFP. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Bestman, Jennifer; Cline, Holly (2022). CIL:36916, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0R49QMT
Type
image
Identifier
ark:/20775/bb8915251n
Language
No linguistic content
Subject
Morphogenesis of a branching structure Neuron development Dendrite Cytoplasm CNS neuron (sensu Vertebrata) Axon Neuronal cell body Xenopus laevis Cell Image Library Group ID: 9208
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