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Image / CIL:10102, Homo sapiens, epithelial cell

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Title
CIL:10102, Homo sapiens, epithelial cell
Creator
Liu, Allen
Schmid, Sandra L
Contributor
Liu, Allen
Schmid, Sandra L.
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Creative Commons Public Domain Dedication
Constraint(s) on Use: This work may be used without prior permission.
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description
Cultured retinal pigment epithelial cells immunofluorescently labeled for clathrin (green) and nucleus (blue). The cells were fixed in 2% PFA and 0.5% Triton X-100 for 2 minutes followed by post-fixation 4% PFA. Clathrin was detected with X22 primary antibody and secondary FITC antibody. The nucleus was detected with DAPI staining. Images were collected on an Olympus IX-71 epifluorescence microscope using a 100X 1.4 NA objective with 4.500ms exposure for clathrin and 50ms exposure for DAPI (67nm/pixel).
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Liu, Allen; Schmid, Sandra L. (2021). CIL:10102, Homo sapiens, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0C24VMG
Type
image
Identifier
ark:/20775/bb91539773
Language
No linguistic content
Subject
ARPE-19
Epithelial cell
Clathrin coat
Nucleus
Homo sapiens

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