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Description
This is a section of methanol-fixed, cryostat-sectioned (10 micrometers thick) adult mouse cervical dorsal root ganglion, labeled with antibodies to peripherin (green) and neurofilament protein NF-M (red) components of the intermediate filament cytoskeleton. Note that small diameter neurons and small-caliber fibers, seen in the lower half of the image, are enriched in peripherin. Large-size neurons and thick-caliber fibers are enriched in NF proteins. Microscope - Zeiss Axioplan I Illumination - Mercury arc lamp Objective - 40X oil/NA 0.9 Intermediate lens between obj and camera - 0.63X Camera - Axiocam MRM CCD Filters: 1. Ex 485/20 BP, Em 515-565 BP 2. Ex 560/40, Em 630/60 Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Parysek, Linda (2021). CIL:111, Mus musculus, sensory neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00R9NMC
Type
image
Identifier
ark:/20775/bb9221932z
Language
No linguistic content
Subject
Structural constituent of cytoskeleton Intermediate filament-based process Sensory neuron Neurofilament Mus musculus
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